Quite often, you will come across news about sports scandals involving top of the line sportsmen accused of doping. Doping is a menace in sports today as some athletes often use performance-enhancing drugs to take unfair advantage in competition. When a scandal surfaces, the sportsman/ woman in question has to undergo a range of tests of blood and urine samples to detect the presence of banned substances in their body. This is an ideal case when a High-Performance Liquid Chromatography Instrument (HPLC) comes handy to analyze the sample to separate the components in it so that the truth comes out. The scope of use of HPLC is widening by the day, and it has a broad range of applications in our lives that we are often not aware of. Let us look at some instances where HPLC plays a significant role in our lives.
Facilitating criminal investigation
A criminal investigation is about not only brains and brawns, but much science is involved in it. Scientific analysis of different kinds of samples obtained from the crime scene plays a critical role in criminal investigation. Among various scientific methods used for analysis of samples, chromatography holds the center stage. Chromatography is widely used in analyzing liquid samples to detect the components present in the compound. In this method, different elements are isolated from the compound that enables its quantification. It helps to detect alcohol levels as well as the presence of toxic elements in blood.
For pathological testing
Pathological testing that we all undergo at some time or the other is another area where we find the use of HPLC in our lives. As health consciousness has increased, more and more people undergo regular medical checkup for which different kinds of pathological test are done. The same happens during medical treatment. Since the aim of testing pathological samples is to detect the presence of different elements in a compound together with its levels, liquid chromatographic tests are most useful for precise results.
Detecting food adulteration
To find out whether or not food is adulterated, the use of HPLC is well known. Since testing by HPLC helps to pin point the various elements present in a food sample, it becomes easy to identify the unwanted element unscrupulous people add to food. If you want to determine the purity of food items, then also you must test it by HPLC that enables detection of impurities in the sample. Chromatographic testing helps to overcome the limitations of other testing methods.
Chromatography saves lives
All of us remember the menace of Ebola virus that created havoc in 2013 in countries like Liberia, Guinea, and Sierra Leone. Had it not been for HPLC, detecting the deadly virus and containing it would be a distant dream. By using chromatography, scientists were able to identify antibodies that could neutralize the Ebola virus.
The advancements that have happened in fighting HIV/AIDS owe a lot to the contribution of HPLC. It has provided more insights to scientists that have given very positive results in controlling the dangers of the disease.